Cureline Human Biospecimen CRO
Olga Potapova, Ph.D., Scientific Director
290 Utah Avenue, Suite 300
South San Francisco, CA 94080
Direct 1-650-875-6400 x 221
Sample types available
Wide range of human biospecimens including fresh, frozen, and fixed (FFPE) tissues, biofluids (blood, serum, plasma, bone marrow, bronchoalveolar lavage, spinal cord fluid, etc.), cell fractions, and primary cell cultures from patients with major indications, normal donors, and postmortem.
FFPE blocks: A piece of human tissue prepared by a certified medical pathologist is submerged in 10% neutral-buffered formalin for maximum of 24 hours and embedded in immunohistochemical-grade paraffin. The target FFPE specimen size is 0.5 x 1 x 1 cm, but it can vary significantly depending on the nature of the disease and tissue type. The fixation agent and the embedding media can be customized upon request.
Frozen tissue: Small pieces of tissue prepared by a certified medical pathologist are snap-frozen in liquid nitrogen usually within 20–30 minutes after the surgical excision or 4–12 hours postmortem in autopsy cases. A standard sample weighs 0.5–2 g on average and is supplied in a standard cryovial. An embedding media can be added upon request.
Cell fractions: Cureline research team has developed protocols for preparation of primary human viable cells and snap-frozen cell pellets for in vitro functional studies and protein- and RNA-expression analysis.
Our scientists can prepare viable primary cells and frozen cell fractions from tumor and normal tissues, blood, and bone marrow. If you would like to implement your custom protocol, please contact Cureline's Scientific Director, Olga Potapova, Ph.D., to discuss your project specifications.
Primary cell lines: The prepared primary cell cultures maintain their differential state and can be used as an in vitro model system to study relevant disease changes and susceptibility to new drug candidates.
Our scientists can prepare primary cell lines from most tissues using our standard protocols. If you would like to implement a custom project, please contact Cureline's Scientific Director, Olga Potapova, Ph.D., to discuss your project specifications.
Serum and Plasma: All specimens are obtained during routine laboratory tests performed as a part of prescribed patient treatment plans. The samples are quickly frozen and stored at –80°C. Each sample is provided with a clinical data set including age, gender, medical history, and treatment. Protocols for prospective collections are available and can be customized to your specific study needs. All specimen collection is conducted under an IRB-approved research protocol, offering a valuable resource for clinical research organizations and diagnostic testing companies.
Data available for each sample
All human specimens provided by Cureline are properly consented, deidentified, and selected with accurate clinical diagnosis and therapy regimen by certified medical professionals. Standard pathology report summary and treatment history. Follow-up is available upon request.
If requested, a custom clinical report form is developed and implemented in prospective procurement studies. A detailed pathology report from a U.S.-board-certified medical pathologist and hematoxylin and eosin stain slides can also be provided upon request.
Quantity of available samples
Cureline Biorepository contains about 100,000 specimens. We also have collaborative access to major clinical repositories through our clinical network.
Who can request samples
Any qualified researcher (material transfer agreement with the employer is required).
Standard price list and individual quotation for custom projects.
How to request samples
Fill out the form at: http://www.cureline.com/downloads/prq.pdf
Request online: http://www.cureline.com/contact-cureline.html
Requirements associated with sample use
Research use only.
Ongoing sample collection?
No publications pertaining to MS or related disorders listed as of March 26, 2012.