Human Brain Tissue Bank, Budapest
Department of Anatomy, Semmelweis University, Budapest, Hungary
Note: The Human Brain Tissue Bank is a member of BrainNet Europe.
Department of Anatomy
Tűzoltó utca 58, Budapest, 1094
Tel. 36-1-215-6920 ext. 3605
Sample types available
HBTB has samples with very short postmortem delay. Most of the brains were removed from the skull and frozen within 2 hours after death. These brains were from the Department of Forensic Medicine of Semmelweis University from persons who died suddenly in public places or in traffic accidents. Additionally, we have brains of suicide victims and some brains from those who died with neurological diseases with somewhat longer (2-6 hours) postmortem delay.
HBTB also has brain samples (mainly from the cerebral cortex) with zero-hour postmortem delay, i.e., tissue samples were taken during neurosurgery and frozen on dry ice right in the surgical room.
We do not store whole brains or large brain pieces; all of our samples are microdissected. The whole procedure is done on frozen brains, and the samples are kept at –70°C. Detailed information about dissection methods can be found at http://humanbraintissuebank-budapest.sote.hu/.
Data available for each sample
Date of death
Cause of death
Date of dissection
Quantity of available samples
Macrodissected brain areas (in 2.0-ml Eppendorf tubes)
50 different brain regions (representative for the whole brain):
100-mg to 2-g tissue weight
Microdisssected brain nuclei and areas (in 1.5-ml Eppendorf tubes)
130 different brain regions:
100-μg to 100-mg tissue weight
Topographically oriented brain tissue blocks
63 different brain regions in about 10 × 20 × 20 mm size ready for cryostat sectioning and in situ hybridization histochemistry.
Who can request samples
Qualified researchers or research laboratories worldwide.
The tissue samples are made available without charge to qualified researchers or research laboratories worldwide.
How to request samples
The tissue samples are made available after signing a statement about scientific collaborations with the HBTB (http://humanbraintissuebank-budapest.sote.hu/dokument/FormNo1.pdf) and a declaration of the proper use of human samples strictly for research studies (http://humanbraintissuebank-budapest.sote.hu/dokument/FormNo2.pdf).
Requirements associated with sample use
The dissected human samples are for medical research examinations. The materials must be studied in the course of an approved research project, which has scientific aims and is devoid of any commercial profit for the researchers involved and for their respective institutions. The samples will not be provided to anybody else.
The fate of the microdissected samples, including their complete utilization, should be reported to the HBTB. If the samples are not used completely, the remaining portions should be buried or resubmitted to the HBTB.
Ongoing sample collection?
No publications pertaining to MS or related disorders listed as of April 27, 2012.